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Srmb

Aug 14, 2020

SrmB, one of the five Escherichia coli DEAD-box proteins, participates in the assembly of the large ribosomal subunit notably by facilitating the incorporation of L13, one of the ribosomal proteins that bind 23S rRNA earliest. Previously, we showed that SrmB is tethered to nascent ribosome through interactions with L4, L24 and the region from

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    srmb

    Sep 04, 2009 The srmB-TAP fusion gene was constructed by fusing two PCR fragments carrying the srmB ORF plus 212 nt upstream sequences, with the TAP tag ( Supplementary Data). The product was cloned into the BamHI site of either pCL1920 ( 13) or pACYC184

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    srmb

    ATP-dependent RNA helicase SrmB UniRule annotation (EC: 3.6.4.13. UniRule annotation ) Gene names i: Name:srmB UniRule annotation. Imported. ORF Names: ACS84_21980 Imported, BS585_11615 Imported, C9I78_13480 Imported, EHC69_02760 Imported, HKB21_23040 Imported, I5M77_11170 Imported, JCT83

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    Aug 22, 2011 The srmB + cells carrying pNK, which grow faster than ΔsrmB cells even in the presence of suppressors, were used as a reference. The ΔsrmB cells containing pNK or the different suppressors (these cells, which are Tet R, Amp R, are referred to as the ‘tested cells’) and the reference cells (Amp R) were first separately grown to saturation

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    srmb

    RNA helicases play various roles in ribosome biogenesis depending on the ribosome assembly pathway and stress state of the cell. However, it is unclear how most RNA helicases interact with ribosome assembly intermediates or participate in other cell processes to regulate ribosome assembly. SrmB is a

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  • srmb
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    Sep 04, 2009 Similarly, the srmB-CBP fusion gene was cloned into NdeI–XhoI sites of pHL9-pET28a-HisTev (Supplementary Data), a pET28 derivative kindly provided by H. Le Hir. Plasmid pET11c-TthL4 encoding Thermus thermophilus L4 was a gift of M. Garber, Pushchino, Russia

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  • srmb
    srmb

    Interacts with the 50S ribosomal subunit. Forms a complex with the 50S ribosomal proteins L4 and L24, and a region near the 5'-end of 23S rRNA. SrmB, a DEAD-box helicase involved in Escherichia coli ribosome assembly, is specifically targeted to 23S rRNA in vivo

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  • srmb
    srmb

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    srmb

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